alexa Analytical Determination of Lisinopril Using UV Spectrophotometer and HPLC: An Overview | Open Access Journals
ISSN: 2329-6798
Modern Chemistry & Applications
Make the best use of Scientific Research and information from our 700+ peer reviewed, Open Access Journals that operates with the help of 50,000+ Editorial Board Members and esteemed reviewers and 1000+ Scientific associations in Medical, Clinical, Pharmaceutical, Engineering, Technology and Management Fields.
Meet Inspiring Speakers and Experts at our 3000+ Global Conferenceseries Events with over 600+ Conferences, 1200+ Symposiums and 1200+ Workshops on
Medical, Pharma, Engineering, Science, Technology and Business

Analytical Determination of Lisinopril Using UV Spectrophotometer and HPLC: An Overview

Safila Naveed*
Faculty of Pharmacy Jinnah University for Women, Pakistan
Corresponding Author : Safila Naveed
Faculty of Pharmacy Jinnah University for women 5-C North Nazimabad
Tel: 009-21-366-324
E-mail: [email protected]
Received July 28, 2014; Accepted September 18, 2014; Published September 23, 2014
Citation: Naveed S (2014) Analytical Determination of Lisinopril Using UV Spectrophotometer and HPLC: An Overview. Mod Chem appl 2:137. doi: 10.4172/2329-6798.1000137
Copyright: 2014 Naveed S. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Related article at
DownloadPubmed DownloadScholar Google

Visit for more related articles at Modern Chemistry & Applications


Present review article determine the analytical methods for the quantitative determinations of lisinopril (ACE Inhibitor) by UV spectrophotometery and High-Performance Liquid chromatography. Pharmaceutical analysis of lisinopril requires effective analytical procedures for QC quality control analysis in Pharmaceuticals dosage formulations and human serum. An extensive survey for determination of LSP compiled from the research articles published in various pharmaceutical and analytical chemistry Journals. This appraisal illustrate that majority of the HPLC methods reviewed are based on the quantitative analysis of drug in Active Pharmaceutical ingredients (API), formulations, biological fluids such as serum and plasma and they are appropriate for therapeutic monitoring of drug, for pharmacokinetic purpose.

Method development validation; High Pressure Liquid Chromatography (HPLC); Lisinopril
Lisinopril (S)-1-[N2-(1-carboxy-3-phenyl propyl) -L- lysyl] -L- proline dihydrate a lysine analogue of enalaprilate, the colour of lisinopril is a white to off-white, state crystalline, no smell and powder having molecular weight of 441.52 and molecular formula C21H31N3O5.2H2O. It is soluble in water and sparingly in methanol, differs from captopril by lacking the sulfhydryl group. It is indicated for the treatment of hypertension, heart failure and acute myocardial infraction. It is indicated as adjunctive therapy in the management of heart failure in patients who are not responding adequately to diuretics and digitalis. It is used to treat hypertension, Myocardial Infarction (MI), heart failure and diabetic nephropathy or retinopathy (Figure 1).
Analytical methods
Assay method for any drug is very significant for pharmaceutical industries and it is always desirable to select and develop simple, least time consuming, precise, accurate and economical method for the determination of drugs in API pharmaceutical dosage forms and pathological samples like blood and serum. Analytical data are used to screen the potential drugs in biological samples, support formulation studies, aid in the development of drug syntheses, monitor the (API) bulk pharmaceuticals and finished products and also test final products for release [1].
Methods of analysis for lisinopril
There are different methods of analysis for lisinopril drug described in literature as,
• Combination of different methods or techniques
• Spectro fluorimetric methods for analysis by reaction modifications
• Electrophoresis /capillary electrophoresis methods
• ( HPLC) High Performance Liquid Chromatographic methods
• UV-visible Spectroscopic methods
• Miscellaneous Methods
Reported methods of analysis
There are numerous methods available for lisinopril analysis in single component or multi component formulation using spectrophotometry [2-4], gas-liquid chromatography [5] capillary electrophoresis and polarography [6,7]. Several High pressure liquid chromatography HPLC methods have been used for the analysis of lisinopril in human plasma [8-10]. Sagirli et al. [11] developed HPLC method for analysis of lisinopril in human plasma and urine at 477 nm. Linear quantitative response was generated over a concentration range of 5-200 ngmL-1 and 25-1000 ngmL-1 for plasma and urine samples.
However, all these methods required laborious experimental work high consumption of organic solvents and these methods were developed on single column. Our research group has worked on HPLC methods for the quantitation of inhibitors as captopril, enalapril and lisinopril alone and in combination with fosinopril and diclofenac sodium [12-16] in bulk drug, pharmaceutical formulations and serum. Sultana et al. [15] have also reported simultaneous methods for the determination of various ACE inhibitors with co-administered drugs as lisinopril with H2 antagonists [17], NSAIDs [18] and with statins [19].
Lisinopril belongs to ACE Inhibitor therefor several methods simultaneously discovered of these ACE Inhibitors including captopril with H2-receptor antagonists [20] diuretics [21] and statins [22]. Enalapril maleate has been simultaneously determined with hydrochlorothiazide and furosemide commonly used diuretics [23], commonly used NSAIDs [24], anti diabetic drugs [25], cholesterol lowering agent statins [26] and with metformin, glibenclamide and glimepride [27] in bulk material, pharmaceutical formulations and human serum using RP-HPLC. Spectrophotometric methods after derivatization of lisinopril and other techniques has been describe previously [3,6,28-33].
Patients diagnosed with hypertension are prescribed multiple medications for therapy which increasing the risk of drug interactions and side effects [34-36]. In this article UV spectrophotometry and HPLC methods for the estimation of lisinopril in (API) active material, formulations and biological samples are reviewed alone or in combination with other drugs [37,38]. High pressure liquid chromatography HPLC methods required expensive equipment, laborintensive sample preparation procedure and the personal skilled in chromatographic techniques [39]. Most of the HPLC methods reviewed have the potential application to clinical research of multi-drug pharmacokinetics, drug combination studies and also for interactions studies.

Figures at a glance

Figure 1
Select your language of interest to view the total content in your interested language
Post your comment

Share This Article

Relevant Topics

Recommended Conferences

Article Usage

  • Total views: 11984
  • [From(publication date):
    November-2014 - Jun 25, 2017]
  • Breakdown by view type
  • HTML page views : 8110
  • PDF downloads :3874

Post your comment

captcha   Reload  Can't read the image? click here to refresh

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version