In the second half of the 20th century, progress in cell biology was far behind molecular biology. Even books entitled “Cell biology" were mostly devoted to molecular interactions rather than the life, structure and physiology of the cell.
Imaging is a key element of modern research as it enables us to directly probe the spatio-temporal distribution of biological processes as we seek to understand their function.
In recent years there has been a paradigm shift towards quantitative imaging. At the forefront of this is high-content screening (HCS) microscopy, a technology that facilitates image acquisition and analysis on a high-throughput scale.
Cells cultured in two-dimension (2D) often stick to the flat bottom of the dish, which can cause misrepresentation of cell morphology, as well as a compromise in cell function.
Microscopy is a booming area of research for all involved parties: cell biologists, physicists, mathematicians, engineers and sell persons.
No imaging is possible without an appropriate sample. The result will be only as good as the weakest part of the whole process of sample preparation and imaging.