Journal of Hematology & Thromboembolic Diseases

ISSN: 2329-8790

Journal of Hematology & Thromboembolic Diseases
Make the best use of Scientific Research and information from our 700+ peer reviewed, Open Access Journals that operates with the help of 50,000+ Editorial Board Members and esteemed reviewers and 1000+ Scientific associations in Medical, Clinical, Pharmaceutical, Engineering, Technology and Management Fields.
Meet Inspiring Speakers and Experts at our 3000+ Global Conferenceseries Events with over 600+ Conferences, 1200+ Symposiums and 1200+ Workshops on Medical, Pharma, Engineering, Science, Technology and Business

Hematotoxicity of Bacillus thuringiensis as Spore-crystal Strains Cry1Aa, Cry1Ab, Cry1Ac or Cry2Aa in Swiss Albino Mice

Visit for more related articles at Journal of Hematology & Thromboembolic Diseases
 

Abstract

Formulated and sporulated cultures of Bacillus thuringiensis (Bt) have been widely used against insect pests, but after the advent of genetically modified plants expressing δ-endotoxins, the bioavailability of Cry proteins has been increased. For biosafety reasons their adverse effects should be studied, mainly for non-target organisms. Thus, we evaluated, in Swiss albino mice, the hematotoxicity and genotoxicity of four Bt spore-crystals genetically modified to express individually Cry1Aa, Cry1Ab, Cry1Ac or Cry2A, administered alone by gavage with a single dose of 27 mg/Kg, 136 mg/Kg or 270 mg/Kg, 24 h, 72 h or 7 days before euthanasia. Binary combinations of these four spore-crystal proteins were also assayed at 270 mg/Kg with a single administration 24 h before euthanasia. Control mice received filtered water or cyclophosphamide at 27 mg/kg. For hematotoxicity evaluations, blood samples were drawn by cardiac puncture and processed in a multiple automated hematology analyzer; for genotoxicity analyses, micronucleus test was carried out in mice bone marrow cells. Spore-crystal administrations provoked selective hematotoxicity for the 3 exposure times, particularly for erythroid lineage. A significant reduction in bone marrow cell proliferation demonstrated cytotoxic but not genotoxic effects. These effects persisted for all exposure times, becoming more evident at 7 days.Similar results were observed for binary combinations at 24 h, suggesting that further studies are required to clarify the mechanism involved in the hematotoxicity found in mice, and to establish the toxicological risks to non-target organisms, especially mammals, before concluding that these microbiological control agents are safe for mammals.

Select your language of interest to view the total content in your interested language
Post your comment
Share This Article
   
 
   
 
Relevant Topics
Disc Acute Lymphoblastic Leukemia
Disc Acute Megakaryocytic Leukemia
Disc Acute Myelomonocytic Leukemia
Disc Acute Myleoid Leukemia
Disc Aleukemic Leukemia
Disc Anemia
Disc AnticoagulantsDrugs
Disc Antiphospholide Antibody Syndrome
Disc Autoimmune Disorder
Disc Autoimmune Haemolytic Anaemia
Disc B-cell Lymphoma
Disc Blood Cancer
Disc Blood Group
Disc Blood Lymphocytes
Disc Body Mass Index
Disc Bone Marrow Disease
Disc Cardiac Diseases
Disc Chronic Lymphocytic Leukemia
Disc Chronic Myleloid Leukemia
Disc Critical Care
Disc Dendritic Cells
Disc Eosinophilia
Disc Family Care Nurse Practitioner
Disc Feline Leukemia Complex
Disc Gastrointestinal Cancer
Disc Haematological Disorders In Children
Disc Haemoglobin
Disc Haemolytic Anaemia
Disc Haemolytic Disease of the Newborn
Disc Haemostasis
Disc Hairy Cell Leukemia
Disc Hepatology
Disc Hypertension
Disc Immunology
Disc Immuoglobulins
Disc Infectious Diseases
Disc Iron Therapy
Disc Juvenile Myelomonocytic Leukaemia
Disc Laboratory Haemostasis
Disc Leukemia
Disc Leukemia Drugs
Disc Leukopenia
Disc Lupus
Disc Lymph
Disc Lymphocytosis
Disc Lymphoma Cancer
Disc Lymphoma Symptoms
Disc Lymphosarcoma
Disc Macrophages
Disc Mast Cell Leukemia
Disc Microbial Pathology
Disc Myelogenous Leukemia
Disc Myeloproliferative Disorders
Disc Neurourology
Disc Neutropenia
Disc Neutrophilic Leukocytosis
Disc Pediatric Leukemia
Disc Plasma Cell Disorder
Disc Plasma Cell Leukemia
Disc Polycythemia Vera
Disc Preventive Care
Disc Primary Care
Disc Prothrombin Time
Disc Pulmonary Diseases
Disc Red Blood Cells
Disc Sickle Cell Disease
Disc Sickle Erythrocytes
Disc Spleen Cancer
Disc Splenectomy Haematological Disease
Disc Systematic Lupus Erythematous
Disc Thrombocythemia
Disc Thromboembolism
Disc Trauma
Disc Tumours
Disc White Blood Cell
 
Recommended Journals
Disc Internal Medicine: Open Access
Disc Journal of Leukemia
Disc Journal of Blood Disorders & Transfusion
  View More»
 
Article Tools
Disc Export citation
Disc Share/Blog this article
 
Article usage
  Total views: 13409
  [From(publication date):
April-2013 - Aug 30, 2016]
  Breakdown by view type
  HTML page views : 9625
  PDF downloads :3784
 
 
 

Post your comment

captcha   Reload  Can't read the image? click here to refresh

OMICS International Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
 
 
OMICS International Conferences 2016-17
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings
 
 

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

agrifoodaquavet@omicsinc.com

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

clinical_biochem@omicsinc.com

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

business@omicsinc.com

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

chemicaleng_chemistry@omicsinc.com

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

environmentalsci@omicsinc.com

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

engineering@omicsinc.com

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

generalsci_healthcare@omicsinc.com

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

genetics_molbio@omicsinc.com

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

immuno_microbio@omicsinc.com

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

omics@omicsinc.com

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

materialsci@omicsinc.com

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

mathematics_physics@omicsinc.com

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

medical@omicsinc.com

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

neuro_psychology@omicsinc.com

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

pharma@omicsinc.com

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

social_politicalsci@omicsinc.com

1-702-714-7001 Extn: 9042

 
© 2008-2016 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version