|Urea; Thiourea; Sodium carbonate; Magnesium sulphate; Biofield treatment; Fourier transform infrared spectroscopy; Ultraviolet-visible spectroscopy
|Pharmaceutical industries are an important component of health care systems which are largely driven by scientific discovery and development of various chemical and biological agents for human and animal health. The pharmaceutical industry is based primarily upon the many organic and inorganic chemicals, which are used as raw materials, serve as reactants, reagents, catalysts, counter ions and solvents. However these chemicals exhibit a wide range of pharmacological activity and toxicological properties . Although the pharma industries are dominated by organic compounds and drugs, the inorganic compounds also focus their attention due to their therapeutic potential such as neurological, anticancer, antimicrobial, antiulcer, antiviral, anti-inflammatory, cardio vascular and insulinmimetic agents. Moreover, inorganic compounds also play an important role as counter ions in drugs, which influence the solubility, stability, and hygroscopicity of active pharmaceutical ingredients . The compounds selected in this study for biofield treatment are urea, thiourea, sodium carbonate and magnesium sulphate, which have wide applications in pharmaceutical industry.
|Urea, a white crystalline powder is commonly used in denaturing and solubilising proteins in the biopharmaceutical industry. It serves an important role in the metabolism of nitrogen-containing compounds by animals and is the main nitrogen-containing substance in the urine of mammals. It is small hydrophilic molecule, present in all taxa, and widely used as protein denaturant in in vitro unfolding/ refolding experiments . It is also used clinically as emollient and keratolytic agent in treatment of skin related diseases .
|Thiourea is an organosulfur compound which is utilized in organic synthesis of various compounds and pharmaceuticals like sulfathiazole’s, thiouracils, tetramizole and cephalosporins. Moreover, it was also used as thyroid depressant during 1940s [5,6].
|Sodium carbonate, commonly known as washing soda, is sodium salt of carbonic acid. Naturally it exists in the form of crystalline heptahydrate; however it readily effloresces to form a white powder which is monohydrate . Sodium carbonate (Na2CO3) is a food additive and used as carbonating agent, anti-caking agent, raising agent, and stabilizer. Its activities are also reported as an alkalizing agent, used in lotion or bath in the treatment of scaly skin in pharmaceuticals .
|Magnesium sulphate is commonly known as Epsom salt, and used both externally and internally in pharmaceutical preparation. In addition, Epsom salt is also used as bath salts and for isolation tanks. Oral magnesium sulphate is commonly used as a saline laxative or osmotic purgative. Magnesium sulphate is the main compound for preparation of intravenous magnesium [9,10]. In all these four compounds, stability plays a crucial role in pharmaceutical preparations, which is directly related to its structural and atomic bonding properties. Currently, in pharmaceutical industries stability of these compounds is modulating through altering temperature and pH conditions . Thus, it is important to evaluate an alternate strategy, which could alter the structural and bonding properties and that can affect the stability in these compounds.
|Biofield is the scientific term for the biologically produced ultrafine electromagnetic energy field that can function for regulation and communication within the organism . It is already demonstrated that electrical current exists inside the human body in the form of vibratory energy particles like ions, protons, and electrons and they generate magnetic field in the human body [13,14]. This electromagnetic field of the human body is known as biofield and energy associated with this field is known as biofield energy [15,16]. Thus, a human has the ability to harness the energy from environment or universe and can transmit the energy into any living or non-living object around this Globe. The object(s) always receive the energy and respond into useful way via biofield energy. This process is termed as biofield treatment. Mr Trivedi’s biofield treatment is recognized as an alternate approach to alter the several physical and structural properties of metal powder at atomic level [17-21]. The biofield treatment has also transformed the characteristics in several other fields like biotechnology [22,23], microbiology [24,25], and in agricultural science [26,27].
|IR spectroscopy which deals with the infrared region and UVVis spectroscopy which deals with ultraviolet-visible spectral region of the electromagnetic spectrum are used in analytical chemistry for the quantitative determination of different analytes, such as transition metal ions, highly conjugated organic compounds, and biological macromolecules . They can provide analytically useful information on a large variety of compounds, ranging from small inorganic ions to large organic molecules . Recently, effect of biofield treatment on ceramic oxide nano powders was studied using infrared spectroscopy, which reported that structural and bond properties were altered after treatment [30-33]. Hence based on the outstanding results achieved on different materials and considering the pharmaceutical applications of these four compounds, the present study was undertaken to evaluate the impact of biofield treatment on the spectroscopic characteristics of urea, thiourea, sodium carbonate, and magnesium sulphate.
|Materials and Methods
|The samples of urea, thiourea, sodium carbonate and magnesium sulphate were procured from Sigma-Aldrich, MA, USA; and each compound was divided into two parts i.e. as control and treatment group. The control samples remained as untreated, and treatment samples were handed over in sealed pack to Mr. Trivedi for biofield treatment under laboratory condition. Mr. Trivedi provided this treatment through his energy transmission process to the treated groups without touching the samples. After that, the control and treated samples of each compound were analysed using Fourier Transform Infrared (FT-IR) spectroscopy and Ultraviolet-Visible (UV-Vis) spectroscopy. Infrared and UV-Vis spectroscopy are particularly useful techniques in identifying organic as well as inorganic structures .
|FT-IR spectroscopic characterization
|The samples were crushed into fine powder for analysis. The powdered sample was mixed in spectroscopic grade KBr (1:20) in an agate mortar and pressed into 3 mm thick pellets with a hydraulic press. FT-IR spectra were recorded on Shimadzu’s Fourier transform infrared spectrometer (Japan) with frequency range of 4000-500 cm-1 at room temperature. The FT-IR spectroscopic analysis of urea, thiourea, sodium carbonate and magnesium sulphate (control and treated) were carried out to evaluate the impact of biofield treatment at atomic and molecular level like bond strength, stability, rigidity of structure etc. The FTIR spectroscopy applied to determine any change in structural and bonding properties due to its ability to characterize the functional group and fingerprint region of very small quantities of samples.
|UV-Vis spectroscopic analysis
|The UV-Vis spectral analysis was measured using Shimadzu UV- 2400 PC series spectrophotometer over a wavelength range of 200-400 nm with 1 cm quartz cell and a slit width of 2.0 nm. This analysis was performed to evaluate the effect of biofield treatment on structural property of different pharmaceutical compounds such as urea, thiourea, sodium carbonate and magnesium sulphate. With UV-Vis spectroscopy it is also possible to investigate electron transfers between orbitals or bands of atoms, ions and molecules existing in the gaseous, liquid and solid phase.
|Results and Discussion
|FT-IR spectroscopic analysis
|Infrared (IR) spectroscopy is based on the vibrations of the atoms in a molecule. When a molecule absorbs infrared radiation, its chemical bonds vibrate and can stretch, contract or bend . FT-IR spectra of control and treated samples of urea are shown in Figure 1. IR spectra of control urea sample showed in plane and out of plane N-H stretching at 3428 cm-1. Other peaks showed C=O stretching at 1684 cm-1; C-N-H bending at 1624 cm-1; C-N stretching at 1458 cm-1 and 1003 cm-1; NH2 bending at 1155 cm-1 and out of plane NH2 bending at 787 cm-1. The spectrum was well supported by literature data . The FT-IR spectrum of treated urea sample showed similar pattern of control IR absorption peaks except N-H stretching peak at 3435 cm-1, C=O stretching peak at 1669 cm-1 and C-N-H bending peak at 1647 cm-1 (Table 1). The shifting of C=O stretching peak towards lower frequency and N-H stretching peak towards higher frequency were may be due to increase of conjugation effect in treated urea molecule. Due to conjugation, there may be increase in bond length of C=O and decrease in bond length of N-H bond of urea. It is already reported that the peak frequency (ν) in IR spectra for any bond is directly proportional to its bond force constant (k) . Also the bond force constant (k) is inversely related to average bond length (r) . Hence, it is presumed that shifting of peak wavenumber corresponding to C=O and N-H bond could be due to change in corresponding bond length after biofield treatment. Data also exhibited that C-N-H bending frequency was shifted towards higher frequency as compared to control sample. It could be due to alteration in bond angle of C-N-H in urea after biofield treatment . As there is occurrence of conjugation effect in treated sample due to biofield treatment, which may lead to increase stability in treated urea as compared to control. Apart from these peaks, small, sharp absorption bands in the region from 4000- 3000 cm-1 and 1800-1600 cm-1 were appeared due to vapour phase water and the predominant CO2 absorption band occurs as a doublet at 2359 cm-1 in both control and treated sample.
|FTIR spectra of control and treated samples of thiourea are shown in Figure 2. The FT-IR spectrum of control thiourea sample showed NH2 asymmetric and symmetric stretching peaks at 3363 cm-1 and 3169 cm-1 respectively. Other peaks were observed for C-N stretching at 1465 cm-1; C=S asymmetric and symmetric stretching at 1412 cm-1 and 730 cm-1 respectively; C-N symmetric stretching peak at 1086 cm-1 and N-C-S bending at 621 cm-1. The peaks in spectrum of control sample were well supported by literature data [40,41]. The FT-IR spectrum of treated thiourea sample showed similar peaks like in control sample except N-C-S bending peak at 660 cm-1 and NH2 asymmetric stretching peak at 3387 cm-1 (Table 1). Hence, the shifting of NH2 stretching peak towards higher frequency as compared to control (3363 cm-1→3387 cm-1) suggest that biofield treatment may reduce the bond length. As described earlier, it ultimately may cause some changes in bond force constant i.e. strengthening of bond which could provide more stability to the compound. Also the change in frequency of N-C-S bending peak suggests that there may be some alteration in bond angle of treated sample as compared to control after biofield treatment. The FT-IR spectra also showed small, sharp absorption bands in the region from 4000-3000 cm-1 and 1800-1600 cm-1 which were appeared due to vapour phase water and a doublet peak at 2359 cm-1 due to CO2 absorption band in both control and treated sample.
|The FT-IR spectra of control and treated samples of sodium carbonate are shown in Figure 3. The FT-IR spectrum of control sample was interpreted regarding the characteristic of IR absorption bands known for carbonate group . IR spectrum of control sodium carbonate showed C-O in plane and out of plane bending peaks at 881 cm-1 and 701 cm-1 respectively and C-O stretching peak at 1445 cm-1. The FT-IR spectrum of control sample also showed the peak at 2943 cm-1 i.e. O-H stretching frequency which could be due to water absorption by sample. The vapour phase water absorption is also evident due to appearance of small, sharp peaks in region of 4000-3000 cm-1. The treated sample also showed O-H stretching peak at 3007 cm-1 other than small and sharp peaks in region of 4000-3000 cm-1 due to water absorption. The spectrum of treated sample showed similar peaks except C-O out of plane bending peak i.e. at 690 cm-1 . The change in C-O bending peak as compared to control sample (Table 2) could be possible due to some change in bond angle of treated sample after biofield treatment.
|FT- IR spectra of control and treated magnesium sulphate samples are shown in Figure 4. The spectrum of control sample showed O-H stretching peak at 3355 cm-1 and O-H bending peak at 1684 cm-1 which may be appeared due to absorption of water molecules by compound. IR spectra of treated sample also showed O-H stretching and bending peaks at 3279 cm-1 and 1660 cm-1 respectively. The absorption of vapour phase water is also evident by appearance of small peaks in region of 4000-3000 cm-1 and 1800-1600 cm-1 in both control and treated sample. Also a predominant CO2 absorption band occurs as a doublet at 2359 cm-1 in both control and treated sample. Other peaks appeared in control sample were mainly due to presence of sulphate group. These were S-O asymmetric stretching peak at 1070 cm-1; S-O symmetric stretching at 983 cm-1 and S-O bending at 621 cm-1 [44,45]. In case of treated sample spectra, similar peaks were observed. The only change observed was in S-O bending peak, which was shifted to higher frequency as compared to control sample (621→ 647 cm-1) (Table 2). It may be due to alteration in bond angle S-O after biofield treatment.
|UV-Vis spectroscopic analysis
|The λmax value corresponding to each control and treated samples are shown in Table 3. In UV spectra of control urea sample, the absorption peak was shown at 201 nm whereas in treated sample the absorption peak was appeared at 220 nm. Shifting of λmax towards higher wavelength in treated sample as compared to control may occur due to increase in conjugation effect in urea molecule. This result was also supported by FT-IR data. Further, it is assumed that conjugation in treated urea may lead to higher stability after biofield treatment . In UV spectra of both control and treated samples of thiourea showed two absorption peaks (202 and 241 nm) and were well supported by literature data . The UV spectra of control and treated samples of sodium carbonate also showed similar pattern of λmax i.e. at 206 nm and which was well supported by literature data . Similarly no change was found in λmax in UV spectra of treated magnesium sulphate as compared to control. These observations suggest that biofield treatment might not make any alteration in chromophore groups present in thiourea, sodium carbonate and magnesium sulphate which are mainly responsible for absorption of light.
|Our group previously reported the impact of biofield energy on physical, thermal and spectroscopic characteristics of various metals and powders [30-33]. The present study also showed the effect of biofield treatment on structural and bonding properties of different pharmaceutical compounds which are used in pharmaceutical industry because of their intrinsic pharmacological action or as intermediate agent. The change in IR frequencies and λmax suggest that due to increase in conjugation effect or force constant between bonds (e.g., urea and thiourea), chemical stability of these compounds might increase after biofield treatment. The increase in stability can improve their shelf life and effectiveness  and make them more suitable to be used in pharmaceutical preparations.
|Altogether, the results of present study showed that there has significant impact of biofield treatment on spectral properties of urea, thiourea, sodium carbonate, and magnesium sulphate. FT-IR data showed significant change in stretching frequencies in treated sample of urea which may be due to increased conjugation effect induced by biofield treatment and attribute to increased stability of treated urea sample. Similarly, a significant change was found in IR peak frequencies related to stretching and bending vibrations of treated samples of thiourea, sodium carbonate and magnesium sulphate which could be due to alteration in bond angle and bond strength after biofield treatment. UV spectroscopic result of urea was also supported by IR data, which suggest that biofield treatment may alter the conjugation effect within the molecule. Thus, it is postulated that biofield treatment can make some alteration at the atomic level, which could further affect the stability of the bonds and hence, the stability of compounds.
|The authors would like to acknowledge the whole team of MGV Pharmacy College, Nashik for providing the instrumental facility.
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